Bsoft program

bhelix

Usage: bhelix [options] input.img output.img

Symmetrizes and projects images with helical symmetry.

Actions:

-normalizeNormalize symmetrized output, use for maps close to symmetry (default not).
-backgroundCalculate new background values.
-project 20Generate projections with this angular step size (over 360 degrees).
-kernel 6,2Reciprocal space projection: kernel size and power.
-elliptical 37,5.5Elliptical distortion: major axis angle and shift (pixels).
-rescale -0.1,5.2Rescale data to average and standard deviation.

Actions for symmetrizing:

-helix 25.3,67.1,2Symmetrize: Helical rise and angle, and dyad axis indicated by 2.
(default 10 angstrom, 45 degrees, no dyad axis).
-rule 237.5,-2,7Symmetrize by selection rule: Helical repeat (angstrom), turns and subunits per repeat.
-super 25.3,67.1,22,15.5Superhelix: Helical rise and angle, and offset in xy plane.
-seam 1.5Symmetrize with the given subunit shift along the seam.

Actions for determining helical parameters:

-findrs 5,8,0.1,10,20,1Find helical parameters in real space:
rise start, end and increment, rotation angle start, end and increment.
-findcc 10,22.4,1.5Find helical parameters by cross-correlation:
rotation angle start, end and increment.
-segment 10,22.4,1.5,18Correlate helical segments: rotation angle start,
end, increment and segment thickness.

Parameters:

-verbose 1Verbosity of output.
-datatype uForce writing of a new data type.
-sampling 1.5,1.5,1.5Sampling (A/pixel; a single value can be given).
-origin 110,50,44Origin for helix (default 0,0,0 or from image).
-zlimits 8,47Range of slices along the helical axis to use (default 0,inf).
-radius 22Radial limit for symmetrization and finding parameters (default from image).
-resolution 15.6,200Resolution limits for projection and cross-correlation (default 0).
-bin 2Binning for finding parameters (default 1).

Output:

-Postscript file.psPostscript output file name.

Bsoft version 2.0.7-20200917 (64 bit)


Generated by bdoc.pl on Mon Oct 26 08:20:11 2020


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