Bsoft program

borient

Usage: borient [options] input.star [input.star]

Determines the orientations and origins of single particles with respect to a reference.
Options -resolution and -bands are mutually exclusive.

Actions:

-mode sccMode: 0,pps: polar power spectrum only (fast, default),
1,scc: polar power spectrum with selected cross correlation.
2,ccc: polar power spectrum with all cross correlation (slow).
3,ori: origin determination by cross correlation.
-CTFApply CTF to projections (default not).
-allReset selection to all particles before other selections (default not).
-side 15Generate side view projections within the given angle from the equator.
-bin 2Binning by given kernel size (default 1).
-prepare 45,12Prepare a set of particle images as 2D references (number and first image).

Parameters:

-verbose 1Verbosity of output.
-select 14Selection number of particles to process (default all selected).
-symmetry C5Point group symmetry.
-angles 2.5,5.2Step size for theta and phi, one value sets both (default 45 degrees).
-sampling 1.5,1.5,1.5Sampling (A/pixel; a single value can be given.)
-resolution 20,360High and low resolution limits (angstrom).
-polarresolution 23.5Resolution limit for in-plane angular alignment (angstrom).
-bands 250,-1,150,1,30,0Bands for analysis in reciprocal space (pairs of numbers: angstrom,flag).
Band with 0: Not used - end flag.
Band with 1: Use for orientation determination.
Band with -1: Use for cross-validation.
-annuli 2,95Real space annular limits (default 0,inf pixels).
-shiftlimit 3.5Limit on origin shift relative to nominal center (default 10% of box edge size).
-rotationlimit 16.7Limit on in-plane rotation change from input orientation (default not applied).
-edge 125Edge mask radius using previous particle origins (default not used).
-fom 0.3Set FOM threshold for selection (default 0).
-nothreadsDo not use threads (default parallel processing).

Input:

-reference file.extInput 3D reference map or projection file.
-mask mask.mrcReal space 2D mask to be applied to projections and particles.

Output:

-output file.starOutput parameter file.
-logWrite particle log files to directory "log".
-ppxWrite temporary particle parameter files to directory "ppx".
-TwoD file.extFile name for 2D real space reconstruction.
-orientedOutput oriented images with file names ending with "_proj.spi".
(must be used with the -TwoD option).

Bsoft version 2.0.7-20200917 (64 bit)


Generated by bdoc.pl on Mon Oct 26 08:20:11 2020


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