Bsoft program

bsegment

Usage: bsegment [options] input.img/input.star [output.img]

Analyzes images for segmentation.

Actions:

-invertInvert density in the image before other operations.
-rescale -0.1,5.2Rescale extracted regions to average and standard deviation.
-blobs 0.45,14,201,1.4Show blobs above threshold (0.45) and set outside minimum (14)
and maximum (201) sizes to value (1.4).
Mask generating actions:
-mass 342kFind threshold associated with this molecular weight (Da)
value can be given in Da, kDa (with k), MDa (with m) or Gda (with g).
Output binary mask (data type byte, values 0 and 1).
-threshold 1.54Report mass calculated from the volume included at this threshold.
Output binary mask (data type byte, values 0 and 1).
-multiple 1.2Generate multiple regions above this threshold.
-kmeans 12,25,0.8K-means segmentation: K, iterations and density:distance ratio.
-innervolume 2.3,15Calculate internal volume below this threshold, output mask frequency.

Selections:

-select 1,5-7Select levels in a multi-level mask.
-combine 1,5-7Combine levels to create a binary mask.
-voxel 15,50,33Select a region around this voxel.

Parameters:

-verbose 7Verbose output.
-datatype uForce writing of a new data type.
-origin 0.8,-10,15.7Set the origin (default from input image).
-sign -1Sign for thresholding (default 0=set by sign of threshold-average;
1=positive density; -1=negative density).
-rho 0.8Protein density (default 0.81 Da/A3, use with -mass).

Input:

-Mask mask.tifInput mask (must be integer and same size as input image).

Output:

-Region region.tifFile name for the excised region.

Bsoft version 2.0.7-20200917 (64 bit)


Generated by bdoc.pl on Mon Oct 26 08:20:11 2020


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