Bsoft program

dcount

Usage: dcount [options] input.star [input2.star...]

Determines the occupancy of components in the maps associated with models.
The maps must have positive density for occupancy determination (use -invert option).

Actions:

-invertInvert density for determination of the threshold.

Parameters:

-verbose 7Verbosity of output.
-componentradius 8.4Set radius for all components.
-shift 40Maximum shift allowed (angstrom, default infinite).
-resolution 50,500Resolution range for cross-correlation (default 0 - 1e6 angstrom).
-rho 0.8Protein density (default 0.81 Da/A3, use with -mass).
-mass 12.645MMolecular mass of the particle (determines the threshold.
-cutoff 0.3Cutoff for voxels above threshold for determining occupancy (default 0.5),
-bins 50Number of bins for histogram of average intensities at the marker locations in all images.
-fit 2Number of fitted binomial distributions to the distribution of occupancy.

Input:

-model model.starInput reference model of expected locations in the particle.
-template templ.pifTemplate map for cross-correlation refinement of component locations.
-Mask shell.mapBinary mask to hide denisity in some regions of the map before determination of occupancy.

Output:

-output model.starModel output file.
-split 3Split models into individual files:
Argument: 1-6: number of digits inserted before extension
Argument: "id": model ID's are used as file names.
-Postscript plots.psPostscript output file.

Examples:

dcount -v 7 -model model_template.star -comp 15.6 -template map_template.pif -shift 55
-resol 50,500 -invert -mass 12.645M -cutoff 50 -bins 20 -fit 2
-output model_occ.star -Postscript model_occ.ps model.star

Bsoft version 2.0.7-20200917 (64 bit)


Generated by bdoc.pl on Mon Oct 26 08:20:12 2020


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