Bsoft program

dmerge

Usage: dmerge [options] input1.star [input2.star...]

Merges corresponding single particle images in a series.
The images can be aligned according to their origin values and the in-plane rotational angle (of the micrograph). You may use the index number of a micrograph in a series, a relative focus level, or an in-plane rotation angle of the micrograph to select a reference micrograph--the one to which the others are aligned. The default is to use the closest-to-focus micrograph in each field-of-view. The default is to use origins from parameter file(s), while origins may also be determined by cross-correlation or obtained from particle image files. Default image output is to one file for every field named '[reference_particle_file_prefix]_merged.img'.

Actions:

-unmerge merged.star	Un-merge, corresponding particles in field get orientations,
	figure-of-merit (FOM), and selection from 'merged.star',
	micrograph rotation angle is accounted for

Parameters:

-verbose 7	Verbosity of screen output, default = 0 (none)
-sampling 1.5	Global sampling (angstrom/pixel)

Parameters for merging:

-origins cross	Get particle origins from parameter files (param, default),
	by cross-correlation (cross), from image files (image),
	or no alignment (none)
-reference near	Reference micrograph: near, nearest-to-focus; far, farthest-from-focus;
	index,1, micrograph index; or angle,0, micrograph rotation angle
	Note: the index option assumes micrographs were entered
	into the parameter file(s) in consistent order

Parameters for unmerging:

-keeporient 0.1	Keep orientation, FOM, & selection if FOM difference >= this value

Output:

-output file.star	Output parameter file
-summed output.img	Write summed images to output.img ( = _micrograph_ids), but only
	if micrograph_ids are <= 6 characters, if not default name is used

Bsoft version 2.0.7-20200917 (64 bit)

Generated by bdoc.pl on Mon Oct 26 08:20:12 2020

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