Protocol Type: physico-chemical assay
Protocol Name: NIST-NCL Joint Assay Protocol, PCC-7: Measuring the Size of Nanoparticles Using Transmission Electron Microscopy (TEM)
Protocol Abbreviation: PCC-7
Protocol Version: 1.1
DOI: http://dx.doi.org/10.17917/C7N7-N938
Protocol File: 20240410_15-41-37-526_NCL_Method_PCC-7.pdf
File Title: PCC-7
Description: Transmission electron microscopy (TEM) has been found to be an excellent tool for characterizing the size of nanoparticles. This assay protocol outlines procedures for sample preparation and the determination of mean nanoparticle size (in projection) using TEM. Although the projected particle size is the primary determinant of the measured particle diameter, other parameters can impact these measurements and influence the measured size. Therefore, guidelines for making successful size measurements in the nanometer-size range are provided, as well as a discussion of relevant standards and data analysis. This protocol can be applied to any suitable TEM instrument with image recording capability.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data