Protocol Type: in vitro assay
Protocol Name: NCL Method GTA-2: Hep G2 Hepatocarcinoma Cytotoxicity Assay
Protocol Abbreviation: GTA-2
Protocol Version: 1.3
DOI: http://dx.doi.org/10.17917/96Z2-NZ61
Protocol File: 20240410_18-18-14-674_NCL_Method_GTA-2.pdf
File Title: GTA-2
Description: This protocol describes the cytotoxicity testing of nanoparticle formulations in human hepatocarcinoma cells (Hep G2), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data