Protocol Type: in vitro assay
Protocol Name: NCL Method GTA-7: Hepatocyte Primary ROS Assay
Protocol Abbreviation: GTA-7
Protocol Version: 1.1
DOI: http://dx.doi.org/10.17917/0WF6-GX40
Protocol File: 20240410_18-35-01-023_NCL_Method_GTA-7.pdf
File Title: GTA-7
Description: This protocol describes the testing of nanoparticle formulations for reactive oxygen species (ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent redox active probe. Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data