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caNanoLab DOI


Protocol Type: Endotoxin

Protocol Name: NCL Method STE-1.1: Detection and Quantification of Gram-Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations Using the End Point Chromogenic LAL Assay

Protocol Abbreviation: STE-1.1

Protocol Version: 3.1


DOI: http://dx.doi.org/10.17917/0DFX-GC30

Protocol File: 20250124_13-01-20-329_NCL_Method_STE-1.1.pdf

File Title: STE-1.1

Description: This document describes a protocol for a quantitative detection of Gram negative bacterial endotoxin in nanoparticle preparations using an end-point Limulus Amebocyte Lysate (LAL) assay. The protocol is based on the QCL-1000 kit manufactured by Lonza Corporation and the USP standard 85 “Bacterial endotoxin test."


Resource Type: Protocol

Data Access: https://general.datacommons.cancer.gov/#/data