Protocol Type: physico-chemical assay
Protocol Name: NCL Method PCC-16: Quantitation of PEG on PEGylated Gold Nanoparticles Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection
Protocol Abbreviation: PCC-16
Protocol Version: 1
DOI: http://dx.doi.org/10.17917/1VHC-MJ13
Protocol File: 20240410_17-09-10-868_NCL_Method_PCC-16.pdf
File Title: PCC-16
Description: This protocol describes a method for the quantitation of polyethylene glycol (PEG) in PEGylated colloidal gold nanoparticles using reversed phase high performance liquid chromatography (RP-HPLC) with charged aerosol detection (CAD). The method can be used to calculate the total PEG on the nanoparticle, as well as the bound and free unbound PEG fractions after a simple centrifugation step.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data