Protocol Type: in vitro assay
Protocol Name: NCL Method ITA-5.2: Analysis of Complement Activation by Single-Plex EIA or Multiplex ELISA
Protocol Abbreviation: ITA-5.2
Protocol Version: 3
DOI: http://dx.doi.org/10.17917/EQCT-3C51
Protocol File: 20240410_19-17-02-617_NCL_Method_ITA-5.2.pdf
File Title: ITA-5.2
Description: This document describes a protocol for quantitative determination of complement activation by an Enzyme Immunoassay (EIA). human plasma is exposed to a test material and subsequently analyzed by EIA for the presence of the complement components C4d, iC3b and Bb. The antibodies specific to these proteins are immobilized on 96 well plates and are obtained from commercial suppliers.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data