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caNanoLab DOI


Protocol Type: in vitro assay

Protocol Name: NCL Method ITA-6.3: Leukocyte Proliferation Assay (Immunosuppression)

Protocol Abbreviation: ITA-6.3

Protocol Version: 2


DOI: http://dx.doi.org/10.17917/M173-MY22

Protocol File: 20240410_19-37-22-337_NCL_Method_ITA-6.3.pdf

File Title: ITA-6.3

Description: This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyte proliferative responses. This assay will allow for measurement of a nanoparticles’ ability to suppress leukocyte proliferation induced by phytohemaglutinin (PHA-M). This protocol is a modification of part B of the NCL ITA-6.1 (formerly NCL ITA-6).


Resource Type: Protocol

Data Access: https://general.datacommons.cancer.gov/#/data