Protocol Type: in vitro assay
Protocol Name: NCL Method ITA-8.2: Analysis of Nanoparticle Chemoattractant Properties Using Label-Free, Real-Time Chemotaxis Assay
Protocol Abbreviation: ITA-8.2
Protocol Version: 1
DOI: http://dx.doi.org/10.17917/QENR-XG24
Protocol File: 20240411_12-08-47-631_NCL_Method_ITA-8.2.pdf
File Title: ITA-8.2
Description: This document provides a protocol for a real-time, quantitative assessment of the chemoattractant capacity of engineered nanomaterials. This assay uses the mouse macrophage cell line RAW 264.7 as a model. The cells are separated from control chemoattractant and test-nanoparticles by an 8 um filter. Cell migration through the filter is monitored using a label-free technology developed by ACEA Biosciences/Agilent, wherein cell attachment to gold electrodes on the underside of the filter results in a change of impedance, which subsequently is converted into a cell index which is proportional to the number of cells that migrated through the filter.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data