Protocol Type: in vitro assay
Protocol Name: NCL Method ITA-18: Human Lymphocyte Activation (HuLa) Assay for Analysis of Immunosuppressive Properties of Nanomaterials
Protocol Abbreviation: ITA-18
Protocol Version: 3
DOI: http://dx.doi.org/10.17917/416C-5429
Protocol File: 20240411_12-52-58-751_NCL_Method_ITA-18.pdf
File Title: ITA-18
Description: Lymphocytes are isolated from human blood, anti-coagulated with Li-heparin, using Ficoll-Paque Premium solution. The blood is obtained from healthy donor volunteers vaccinated with the current season flu vaccine (Fluzone). The isolated cells are incubated with Fluzone in the presence or absence of nanoparticles. After a 72 hour incubation, BrdU is added to cells and the incubation continues for additional 24 hours. At the end of incubation, the cells are fixed and incorporation of BrdU into DNA of proliferating cells is analyzed by ELISA.
Resource Type: Protocol
Data Access: https://general.datacommons.cancer.gov/#/data